Journal: Open Life Sciences

Article Title: Association between TGF-β1 and β-catenin expression in the vaginal wall of patients with pelvic organ prolapse

doi: 10.1515/biol-2022-1058

Figure Lengend Snippet: RT - qPCR primer sequences

Article Snippet: Wuhan Sanying), TIMP2 (Batch No.: A1558, Abcolnal), collagen type I alpha 1 chain (COL1A1; Batch No.: BA0325, Boster), reverse transcription kit (Batch No.: MF166-Plus-01, Juemei), fluorescence quantification kit (Batch No.: MF787-01, Juemei), enhanced RIPA lysate (Batch No.: AR0102-100, Boster), BCA protein quantification kit (Batch No.: AR0146, Boster), protease inhibitor (Batch No.: AR1178, Boster), 5X protein sampling buffer (Batch No.: AR1112-10, Boster), and electrochemiluminescence (ECL) luminescent liquid (Batch No.: MA0186, Meilun).

Techniques: Quantitative RT-PCR, Sequencing

Journal: Open Life Sciences

Article Title: Association between TGF-β1 and β-catenin expression in the vaginal wall of patients with pelvic organ prolapse

doi: 10.1515/biol-2022-1058

Figure Lengend Snippet: (a)–(e) Expression of TGF-β1, β-catenin, MMP2, TIMP2, and COL1A1 in the anterior vaginal wall tissues of patients in the control group. (f)–(j) Expression of TGF-β1, β-catenin, MMP2, TIMP2, and COL1A1 in the anterior vaginal wall tissues of patients in the POP group, respectively. (a)–(j) magnification 50×.

Article Snippet: Wuhan Sanying), TIMP2 (Batch No.: A1558, Abcolnal), collagen type I alpha 1 chain (COL1A1; Batch No.: BA0325, Boster), reverse transcription kit (Batch No.: MF166-Plus-01, Juemei), fluorescence quantification kit (Batch No.: MF787-01, Juemei), enhanced RIPA lysate (Batch No.: AR0102-100, Boster), BCA protein quantification kit (Batch No.: AR0146, Boster), protease inhibitor (Batch No.: AR1178, Boster), 5X protein sampling buffer (Batch No.: AR1112-10, Boster), and electrochemiluminescence (ECL) luminescent liquid (Batch No.: MA0186, Meilun).

Techniques: Expressing, Control

Journal: Open Life Sciences

Article Title: Association between TGF-β1 and β-catenin expression in the vaginal wall of patients with pelvic organ prolapse

doi: 10.1515/biol-2022-1058

Figure Lengend Snippet: (a)–(e) Statistical results of immunohistochemical staining of TGF-β1, β-catenin, MMP2, TIMP2, and COL1A1, respectively.

Article Snippet: Wuhan Sanying), TIMP2 (Batch No.: A1558, Abcolnal), collagen type I alpha 1 chain (COL1A1; Batch No.: BA0325, Boster), reverse transcription kit (Batch No.: MF166-Plus-01, Juemei), fluorescence quantification kit (Batch No.: MF787-01, Juemei), enhanced RIPA lysate (Batch No.: AR0102-100, Boster), BCA protein quantification kit (Batch No.: AR0146, Boster), protease inhibitor (Batch No.: AR1178, Boster), 5X protein sampling buffer (Batch No.: AR1112-10, Boster), and electrochemiluminescence (ECL) luminescent liquid (Batch No.: MA0186, Meilun).

Techniques: Immunohistochemical staining, Staining

Journal: Open Life Sciences

Article Title: Association between TGF-β1 and β-catenin expression in the vaginal wall of patients with pelvic organ prolapse

doi: 10.1515/biol-2022-1058

Figure Lengend Snippet: (a)–(e) Statistical results of TGF-β1, β-catenin, MMP2, TIMP2, and COL1A1 mRNA expression levels in the anterior vaginal wall tissues of control and POP groups.

Article Snippet: Wuhan Sanying), TIMP2 (Batch No.: A1558, Abcolnal), collagen type I alpha 1 chain (COL1A1; Batch No.: BA0325, Boster), reverse transcription kit (Batch No.: MF166-Plus-01, Juemei), fluorescence quantification kit (Batch No.: MF787-01, Juemei), enhanced RIPA lysate (Batch No.: AR0102-100, Boster), BCA protein quantification kit (Batch No.: AR0146, Boster), protease inhibitor (Batch No.: AR1178, Boster), 5X protein sampling buffer (Batch No.: AR1112-10, Boster), and electrochemiluminescence (ECL) luminescent liquid (Batch No.: MA0186, Meilun).

Techniques: Expressing, Control

Journal: Translational Oncology

Article Title: A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response

doi: 10.1016/j.tranon.2025.102349

Figure Lengend Snippet: Clinical information of bladder cancer patients.

Article Snippet: After blocking, added the prepared TIMP2 (MCE, HY-P80356, 1:200) primary antibody to each slice and incubate overnight.

Techniques:

Journal: Translational Oncology

Article Title: A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response

doi: 10.1016/j.tranon.2025.102349

Figure Lengend Snippet: Correlation analysis between TIMP2 and MSC. (A) Thumbnail of TMA for TIMP2 immunohistochemistry. (B) IHC of tissues with high TIMP2 expression and corresponding regions stained with mFIHC (where the gold-colored cells indicated by arrows represent MSCs). (C) IHC of tissues with lowTIMP2 expression and corresponding regions stained with mFIHC. (D) Correlation analysis between TIMP2 expression and MSC proportion in 23 cases of BLCA tissues.

Article Snippet: After blocking, added the prepared TIMP2 (MCE, HY-P80356, 1:200) primary antibody to each slice and incubate overnight.

Techniques: Immunohistochemistry, Expressing, Staining